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Saliva Testing for Hormones

Some ADVANTAGES of saliva testing compared to serum testing:

  • Saliva shows the free, unbound fraction of steroid hormones in the bloodstream-it is these biologically active fractions that are bio-available to the tissues throughout the body. Blood and urine tests measure total levels.
  • Collection times can be more optimal which is very important for baseline hormone testing and assessment of HRT dosing. Note: Some hormones naturally fall by 50-90% during the course of the day so collection time is important. Hormone production is optimal in the morning and less susceptible to variation caused by stress, eating and drinking, and exercise.
  • Painless, noninvasive, simple, safe, and needle-free.
  • Private and convenient for both the patient and doctor.
  • Hormones are stable in saliva at room temperature for 7-10 days.
  • Transport of saliva to laboratory by regular US mail.
  • Less expensive than blood testing.

Estrogens, progesterone, testosterone, DHEA-S and cortisol are routinely measured in saliva. Steroid hormones in the bloodstream are mostly (95-99%) bound to carrier proteins, and in this form are unavailable to target tissues. Only the unbound fraction freely diffuses into tissues, including the salivary gland. Hormone levels in saliva therefore represent the quantity of the hormone that is currently available to target tissues and actively exerting specific effects on the body. Research has demonstrated clear correlations between salivary hormone levels and reported symptoms.

Saliva Sample Volumes

Why do we ask for a larger sample volume? Steroid hormones are secreted in a bursts and pulses by various glands throughout the body. The more saliva that is collected, the more the effect is averaged out, and thus the results become more reliable and of the highest clinical relevance. Read More

Click here to view our Validity Statement for Saliva Testing.

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Saliva Combo Test Kit

For an initial evaluation of overall hormonal status, it is strongly recommended that most comprehensive profiles use both saliva and blood spot collection, which give you the “big picture” of where the hormones may be unbalanced.

Hormones tested:

  • Estradiol (E2)
  • Progesterone (pg)
  • Testosterone (T)
  • DHEA-S (Ds)
  • Cortisol x4 (C)
  • Free T4 (Inactive Thyroid Hormone)
  • Free T3 (Active Thyroid Hormone)
  • TSH
  • TPO Antibodies

Sex Hormones Measured in Saliva:

Choose from the following:

  • Estradiol (E2)
  • Estrone (E1)
  • Estriol (E3)
  • Progesterone (pg)
  • Testosterone (T)
  • DHEA-S (Ds)
  • Cortisol (C)

Dried blood spot is an important part of the minimally-invasive hormone testing. The simplicity of sample collection, stability of samples in storage and transport, and excellent correlation of blood spot assays with serum tests, have made it an ideal method for epidemiological and field research studies for a variety of health conditions in both children and adults.


CardioMetabolic Test

Understand your increased risk for cardiovascular disease and type II diabetes. Proper management of CardioMetabolic risk factors can support your overall wellness and provide effective tools for improved lifestyle and longevity.


Vitamin D Blood Spot Test

Vitamin D deficiency (levels measured below 32 ng/ml) does not have obvious symptoms and can increase your risk for more serious diseases including flu, cardiovascular disease, osteoporosis and multiple sclerosis. In addition, an increased risk for strokes, diabetes (types 1 and 2), depression, and breast and colon cancer are closely linked to low Vitamin D levels.


Thyroid Blood Spot Test

Thyroid levels from Blood Spot Test

  • TSH – Thyroid Stimulating Hormone
  • Free T4 – Thyroxine
  • Free T3 – Triiodothyronine
  • TPO – Thyroid Peroxidase Antibodies
Iodine Test Kit

Dried urine is for testing elements (iodine, selenium, bromine, arsenic and mercury) that directly or indirectly impact thyroid hormone synthesis and intracellular conversion of T4 to T3. Dried urine tests have also been developed for measuring steroid hormones and their metabolites. Urine dried on filter paper produces results nearly identical to liquid urine, but has clear advantages in sample transport, size and stability compared to typical 24-hour urine collections.

Weight Management Test

Many know that maintaining an ideal weight is difficult despite the best efforts with diet and exercise. What most don’t know is that an undetected hormone imbalance can be the missing link – sabotaging your hard work to stay slim. Hormones are key players in regulating weight, metabolism, blood sugar, insulin, and when and where the body stores fat. As we age, shifting hormones trigger numerous symptoms of imbalance – including unexplained weight gain. The good news is that there is a simple, at-home test that can identify whether you’re one of the thousands affected.

Fertility Test

21.4% of women age 30 to 44 struggle with infertility. The primary causes of infertility are physical, including ovulatory failure, tubal damage, and endometriosis. However, 28% of infertility cases remain unexplained. In the absence of a physical cause, many cases of infertility may be explained by something as simple and correctable as hormonal imbalances.

Sleep Balance Profile

Several key hormones rule the sleep-wake cycle, and restful sleep demands on those being in balance. With an estimated 60 million Americans suffering from some degree of insomnia - whether that be early waking or the inability to get to sleep and stay asleep - the need to measure and monitor these hormone levels is paramount.

The first four-point test profile offering a complete diurnal pattern of melatonin and cortisol.

Advantages of ZRT’s Test Method:

  • No more 2:00am wake-ups – morning collection reflects overnight melatonin levels
  • More accurate results because testing does not interrupt sleep
  • Measures cortisol and melatonin at 4 time points throughout the day to provide a true 24-hour sleep-wake pattern
  • Discreet dried urine collection eliminates the inconvenience of jug urine collection